HtrA1 is serine protease which has been identified in the epidermis.1 Although the precise function of HtrA1 in the epidermis is still unclear, some reports have suggested that HtrA1 is involved in programmed cell death, apoptosis and anoikis.2,3
On the other hand, it is generally known that excess apoptosis caused by UVB exposure is involved in the accumulation of cell damage in the skin epidermis, and it adversely affects skin epidermis homeostasis that causes chronic cell inflammation and parakeratosis. However, the precise mechanism underlying how UVB induced apoptosis adversely affects epidermal alterations has not been elucidated. This study aims to clarify the behaviour of HtrA1 in the apoptosis of epidermal cells induced by UVB exposure. Furthermore, we evaluated the efficacy of Japanese maple extract, a potent inhibitor of HtrA1 we found through this study.
Materials and methods
Cell culture
Normal human keratinocytes (NHKs, Kurabo) were purchased from Kurabo, and were cultured in Humedia-KG2 medium (KG2, Kurabo)
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