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Joint development of 3D model of dermal microtissue

During ageing, human skin undergoes profound alterations of its biomechanical properties, and more particularly a loss of elasticity that results in skin sagging.

Dermal elastic fibres represent the primary components that support tissue compliance and
resilience. But as time goes by, their organisation and functionality decline, which makes them a preferred target for cosmetic anti-ageing strategies.

The current 3D bioengineered skin substitutes, which are easily available on the market, are still defective models to study skin elasticity. Indeed, they contain exogenous and artificial matrices that bias the measurement of biomechanical properties in the reconstructed tissue. So, there is a need to develop advanced models to investigate the mechanical structure of a tissue such as human skin.

3D scaffold-free microtissues were developed by Gattefossé laboratories to mimic in vitro an elastic tissue, which is responsible for intrinsic elastic properties of the dermis. To accurately evaluate the elasticity of such skin microtissues, Gattefossé chose BioMeca’s expertise for developing innovative analytical assessment with state-of-the-art technologies.

3D scaffold-free spheroids take advantage of the ability of cells to secrete their own extracellular matrix to ultimately recreate their own microenvironment. This technology enabled Gattefossé to produce in vitro hundreds of 3D microtissues within a few days only using dermal fibroblasts aggregated in ultra-low affinity plates.

The elastic modulus (or Young modulus) was then measured using Atomic Force Microscopy (AFM) and the elastic fibers were visualised by Second Harmonic Generation (SHG) imaging microscopy. Gattefossé and BioMeca thus demonstrated that the 3D spheroid microtissue is a relevant and reliable model with a complex organisation, comprising a dense, mature elastic fibre network sufficiently extensive to mimic in vitro dermal elastic mechanics.

This advanced 3D model has been successfully used to measure the efficacy of EleVastin a novel active ingredient developed by Gattefossé, fighting against age-related loss of skin elasticity. 

See more about Gattefossé

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Upcoming Events

Cosmetic Ingredients & Technology Exhibition Japan (CITE JAPAN)

Pacifico Yokohama Exhibition Hall / North / Annex Hall
19 May – 21 May 2021

in-cosmetics Korea 2021

Hall C, Coex, Seoul, South Korea
14 - 16 July 2021

The Society of Cosmetic Scientists, Singapore - 11th SCSS Suppliers Day, August 2021

Marriott Singapore, Tang Plaza Hotel. 320 Orchard Road
Thursday 26th & Friday 27th August 2021

in-cosmetics Asia 2021

BITEC Bangkok, Thailand
2 - 4 November 2021

NYSCC Suppliers’ Day 2021

Jacob K. Javits Convention Center, New York
10 - 11 November 2021

Access the latest issue of Personal Care Magazine on your mobile device together with an archive of back issues.

Download the FREE Personal Care Magazine app from your device's App store

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